Members

• F. William Luscinskas, Ph.D. - Director
  Brigham & Women's Hospital
  Room 730P
  77 Avenue Louis Pasteur
  Boston, MA 02115
  Tel: 617-525-4337
  fluscinskas@rics.bwh.harvard.edu

Primary Goal

Since its inception in 1978, the primary goal of the Cell Biology Core has been to provide researchers within the Vascular Research Division with a range of well characterized cultured cells from both vascular and non-vascular sources. Core members also work closely with researchers to develop new cell culture systems, as needed, to facilitate the scientific goals of both individual investigators and the Division as a whole.

Functions

• Provides and maintains a variety of well characterized cultured cells
• Quality control testing of key biological reagents
• Provides training in general cell culture methods
• Provides training to new personnel for dealing with biohazardous biological material
• Provides access to a fluorescence microscopy /computerized image analysis system

Protocols

• Bovine Aorta Endothelial Cell
• Bovine Aorta Smooth Muscle Explants
• Enzymatic Dissociation of Rat Mesenteric Artery
• Freezing Adherent Cells
• Harvesting Endothelial Cells From Human Umbilical Cord Vessels
• Subculturing Human Umbilical Vein Endothelial Cells (HUVEC)
• Isolation of Mouse Lung Endothelial Cells (MLEC)/ Mouse Heart Endothelial Cells (MHEC)

Core Facilities

• Cell Biology Core
• Morphology Core
• Imaging Core