Core Facilities
Morphology Core
Members
- David Milstone, M.D. Ph.D. - Director
Brigham & Women's
Hospital
Room 730G
77 Avenue Louis Pasteur
Boston, MA 02115
Tel: 617-525-4308
milstone@rascal.med.harvard.edu
- George Stavrakis
gstavrakis@rics.bwh.harvard.edu
Primary Goal
The primary goal of the Morphology
Core is to make available to the investigators of the Vascular
Research Division the expertise, facilities, techniques and
technical support required for morphologic, immunolocalization
and RNA in situ hybridization studies of cultured
cells and of animal and human tissues at both the light and
electron microscopic level. Additionally, Dr. Milstone and
Mr. Stavrakis provide consultation to investigators within
the Division in interpreting histopathologic changes and in
procedures for selecting and preparing tissues from experimental
animals for morphologic studies.
Functions
- Specimen preparation
- Histopathology
- Immunohistochemistry of microscopic
sections
- Immunohistochemistry of whole mount
specimens
- In situ hybridization
- Electron microscopy
- Immunoelectron microscopy
Facilities
The Morphology Core laboratory
is located on the seventh floor of Harvard's New Research
Building (HNRB), within the Center for Excellence in Vascular
Biology's Vascular Research Division of Brigham & Women's
Hospital. Equipment is available within the Core laboratory,
the Vascular Research Division and the Department of Pathology
for processing tissues and cultured cells for light microscopy,
immunohistochemistry (frozen sections and paraffin embedded
and sections), electron microscopy and immunoelectron microscopy,
embedding in paraffin, photomicroscopy, scanning and transmission
electron microscopy, radioisotopic and non-radioactive in
situ hybridization, and image analysis hardware, software
and consultant expertise.
Consultants
Other
members of the Department of Pathology serve as consultants
to this Core. Dr. Helmut Rennke is highly experienced in electron
microscopy and immunohistochemistry and Dr. Daniel Friend
is an internationally recognized microscopist and cell biologist
with particular expertise in immuno-light and electron microscopy.
Dr. Friend also directs a Morphology Core Facility in the
Department of Rheumatology and Immunology at Brigham and Women's
Hospital (Dr. Frank Austen, Chairman).
References
Click here for Morphology Core selected references. |
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Core Facilities
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